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anti pp38  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti pp38
    Anti Pp38, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 30317 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+pp38/p38+MAPK+Antibody/pm41898405-215-21-47
    Average 99 stars, based on 30317 article reviews
    anti pp38 - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Western Blot:

    Article Title: Mechanisms of Programmed Cell Death in Sodium Iodate-Driven Retinal Degeneration and the Role of DJ-1
    Article Snippet: An amount of 30 μg of protein for retina and RPE lysate were separated on 4–20% Tris-Glycine SDS PAGE (Bio-rad, San Francisco, CA, USA) and transferred on PVDF membrane (Immobilon-FL, Merck Millipore Ltd., Burlington, MA, USA) under wet conditions at a constant 100 volts for an hour. .. Immunoblotting membranes were reacted with the following antibodies: anti-ERK1/2 (rabbit polyclonal, 4695S), anti-phospho ERk1/2 (rabbit polyclonal, 9101S), anti-P38 (rabbit polyclonal, 4511S), anti-pP38 (rabbit polyclonal, 9212S), anti-JNK (rabbit polyclonal, 9252S), anti-pJNK (rabbit polyclonal, 9251S), anti-FOXO1 (rabbit monoclonal, C29H4), and β-actin (mouse monoclonal 8H10D10); all antibodies were purchased from Cell Signaling Technology, Danvers, MA, USA. .. The following secondary antibodies provided by Licor Biosciences (Lincoln, NE, USA) were used: anti-rabbit IRDye ® 680RD and anti-rabbit IRDye ® 800CW.

    Article Title: Mechanisms of Programmed Cell Death in Sodium Iodate-Driven Retinal Degeneration and the Role of DJ-1.
    Article Snippet: An amount of 30 μg of protein for retina and RPE lysate were separated on 4–20% Tris-Glycine SDS PAGE (Bio-rad, San Francisco, CA, USA) and transferred on PVDF membrane (Immobilon-FL, Merck Millipore Ltd., Burlington, MA, USA) under wet conditions at a constant 100 volts for an hour. .. Immunoblotting membranes were reacted with the following antibodies: anti-ERK1/2 (rabbit polyclonal, 4695S), anti-phospho ERk1/2 (rabbit polyclonal, 9101S), anti-P38 (rabbit polyclonal, 4511S), anti-pP38 (rabbit polyclonal, 9212S), anti-JNK (rabbit polyclonal, 9252S), anti-pJNK (rabbit polyclonal, 9251S), anti-FOXO1 (rabbit monoclonal, C29H4), and β-actin (mouse monoclonal 8H10D10); all antibodies were purchased from Cell Signaling Technology, Danvers, MA, USA. .. The following secondary antibodies provided by Licor Biosciences (Lincoln, NE, USA) were used: anti-rabbit IRDye®680RD and anti-rabbit IRDye®800CW.

    Blocking Assay:

    Article Title: Promising effects of astaxanthin on pain-related behaviors and morphine tolerance in a mouse model of neuropathic pain: Possible involvement of Nrf2 and the NMDA receptor subunit NR2B
    Article Snippet: Next, the proteins were transferred (semidry transfer at 25 V for 30 min) to Immune-Blot PVDF membranes (Bio-Rad) and blocked at RT for 1 h with 5 % bovine serum albumin (Sigma‒Aldrich) in Tris-buffered saline containing 0.1 % Tween-20 (TBST). .. Following the blocking step, the membranes were washed with TBST and incubated overnight (4 °C) with the following primary antibodies: rabbit anti-cFos (1:1000, 2250; Cell Signaling, Danvers, USA), anti-Iba-1 (1:500, NBP2-19019; Novus, Abingdon, UK), anti-Gfap (1:10 000, NB300-141; Novus), anti-pp38 (1:1000, 4631; Cell Signaling), anti-pERK1/2 (1:1000, 4370; Cell Signaling), anti-pJNK (1:1000, 4671; Cell Signaling), anti-pNFκB (1:1000, sc-33039; Santa Cruz, Dallas, USA), anti-Sod1 (1:1000, 37 385; Cell Signaling), anti-Nrf2 (1:1000, 20 733; Cell Signaling), anti-NR2B (1:1000, 4212; Cell Signaling) and mouse anti-β-actin (1:1000; SAB1305554-40TST, Sigma). .. Afterward, the membranes were washed with TBST and incubated for 1 h at room temperature with HRP-conjugated anti-rabbit or anti-mouse secondary antibodies (1:5000, Vector Laboratories, Burlingame, CA, USA) diluted in SignalBoostTM Immunoreaction Enhancer Kit (Merck) buffer.

    Incubation:

    Article Title: Promising effects of astaxanthin on pain-related behaviors and morphine tolerance in a mouse model of neuropathic pain: Possible involvement of Nrf2 and the NMDA receptor subunit NR2B
    Article Snippet: Next, the proteins were transferred (semidry transfer at 25 V for 30 min) to Immune-Blot PVDF membranes (Bio-Rad) and blocked at RT for 1 h with 5 % bovine serum albumin (Sigma‒Aldrich) in Tris-buffered saline containing 0.1 % Tween-20 (TBST). .. Following the blocking step, the membranes were washed with TBST and incubated overnight (4 °C) with the following primary antibodies: rabbit anti-cFos (1:1000, 2250; Cell Signaling, Danvers, USA), anti-Iba-1 (1:500, NBP2-19019; Novus, Abingdon, UK), anti-Gfap (1:10 000, NB300-141; Novus), anti-pp38 (1:1000, 4631; Cell Signaling), anti-pERK1/2 (1:1000, 4370; Cell Signaling), anti-pJNK (1:1000, 4671; Cell Signaling), anti-pNFκB (1:1000, sc-33039; Santa Cruz, Dallas, USA), anti-Sod1 (1:1000, 37 385; Cell Signaling), anti-Nrf2 (1:1000, 20 733; Cell Signaling), anti-NR2B (1:1000, 4212; Cell Signaling) and mouse anti-β-actin (1:1000; SAB1305554-40TST, Sigma). .. Afterward, the membranes were washed with TBST and incubated for 1 h at room temperature with HRP-conjugated anti-rabbit or anti-mouse secondary antibodies (1:5000, Vector Laboratories, Burlingame, CA, USA) diluted in SignalBoostTM Immunoreaction Enhancer Kit (Merck) buffer.



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